Determination of Poly(3-hydroxybutyrate) Content in Cyanobacterium Synechocystis sp. PCC 6803 Using Acid Hydrolysis Followed by High-performance Liquid Chromatography

Various photoautotrophic cyanobacteria accumulate intracellular poly(3-hydroxybutyrate) (PHB) granules. This protocol can be used for determining the PHB contents of the cells as % PHB weight per dry cell weight using acid hydrolysis followed by high-performance liquid chromatography (HPLC). This HP...

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Bibliographic Details

Published in:	BIO-PROTOCOL
Main Author:	Janine Kaewbai-ngam
Other Authors:	Aran Incharoensakdi, Tanakarn Monshupanee
Format:	Article
Language:	English
Published:	American Academy of Arts and Sciences 2023
Subjects:	biodegradable polymer synthesis and properties Algal biology and biofuel production Biopolymer Synthesis and Applications polyhydroxybutyrate Synechocystis sp. PCC 6803 cyanobacteria acid hydrolysis PHB granules
Online Access:	https://doi.org/10.21769/bioprotoc.4790
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